4X Laemmli SDS-Sample Buffer (+ Reducing Agent)

DESCRIPTION

The 4X Laemmli SDS-Sample Buffer with the reducing agent 2-mercaptoethanol (β-mercaptoethanol) is used for preparation of samples for SDS PAGE.

Laemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel.

Our 4X Laemmli Sample Buffer can be used with both home-made and commercially available precast protein gels such as Mini-PROTEAN^® and midi Criterion™ gels from BioRad, Novex Tris-Glycine gels from ThermoFisher, and many others.

The 4X SDS-Sample buffer is composed of 250 mM Tris-HCl, 8% Sodium Dodecyl Sulfate (SDS), 40% Glycerol, 0.02% Bromophenol Blue, and 8% Beta-Mercaptoethanol (pH 6.8 ± 0.1).

The final concentrations in 1X SDS Sample buffer are: 62.5 mM Tris-HCl, 2% Sodium Dodecyl Sulfate (SDS), 10% Glycerol, 0.005% Bromophenol Blue, and 2% Beta-Mercaptoethanol (pH 6.8 ± 0.1).

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.

The sample buffer is evaluated and certified for the absence of RNase/DNase and protease activities.

PROCEDURE:

Dilute 1 volume of the 4X sample buffer with 3 volumes of sample solution (Example: add 5 µL of the 4X sample buffer to each 15 µL sample).

For dry or precipitated protein samples, dilute the sample buffer to 1X using deionized water. Then dissolve the protein sample in an appropriate amount of the diluted sample buffer.

Samples are heated in gel loading/sample buffer for either 5 minutes at 100 ᴼC, or 10 minutes at 70 ᴼC to aid in the denaturation. At this point, samples can remain at room temperature if they are to be used immediately or placed at 4 ᴼC or –20 ᴼC for later analysis.

PACKAGING:

Available in 4 different sizes: 1.5 mL, 10 mL, 25 mL, and 50 mL.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact us with your request for customized packaging.

STORAGE TEMP

2-15 ᴼC.

SHELF LIFE

1 years.

NOTES

Molecular/Electrophoresis grade buffer, DNase/RNase, and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 4 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.