DESCRIPTION
10X concentrated Bjerrum Schafer-Nielsen Transfer Buffer is used in semi-dry transfer system in Western blot protocol.
The Buffer is available in two options: With and without SDS. Please see the two buffer compositions in the table below.
Cat. No. | SDS | Composition |
---|---|---|
BSN01-10X-500ML BSN01-10X-1L BSN01-10X-1GAL |
With SDS | 480 mM Tris, 390 mM Glycine, and 13 mM SDS (pH 9.2 ± 0.1) |
BSN02-10X-500ML BSN02-10X-1L BSN02-10X-1GAL |
Without SDS | 480 mM Tris, and 390 mM Glycine (pH 9.2 ± 0.1) |
To prepare a 1X solution, mix one volume of the 10X Bjerrum Schafer-Nielsen Buffer solution with 9 volumes of ultrapure water + the appropriate amount of methanol. The final concentrations in 1X solution (Cat. No.: BSN01-10X): 48 mM Tris Base, 39 mM Glycine, and 1.3 mM SDS (pH 9.2 ± 0.1), and for (Cat. No.: BSN01-10X): 48 mM Tris Base, and 39 mM Glycine (pH 9.2 ± 0.1).
Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.
The buffer is Sterile filtered through 0.22 µm membrane, and autoclaved.
The buffer is evaluated and certified for the absence of DNase, RNase, and protease activities.
PACKAGING:
Available in varied sizes: 500 mL, 1000 mL, and 1 Gallon (3.78 L).
Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.
Minimum order: 1 Bottle.
We accommodate customized packaging requests. Please contact us with your request for customized packaging.
STORAGE TEMP
Room Temperature.
SHELF LIFE
2 years.
NOTES
Molecular Biology / Electrophoresis grade buffer, filter sterile, and DNase/RNase and protease free.
Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.
Minimum order: 1 bottle.
Request a discounted quote for quantities ≥ 4 Bottles.
This product is developed, designed, and sold exclusively for research purposes and in vitro use only.
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