10X Dunn Carbonate Transfer Buffer (pH 9.9) Sterile Filtered, DNase/RNase and Protease Free

DESCRIPTION

The 10X Dunn Carbonate buffer is recommended for transferring basic proteins in Western blotting protocols. It may also increase the efficiency of electroblotting of large-size proteins.

The 10X Dunn Buffer is composed of 100 mM Sodium Bicarbonate (NaHCO₃), and 30 mM Sodium Carbonate (Na₂CO₃), pH 9.9 ± 0.1.

The final Concentrations in 1X solution: 10 mM NaHCO₃, and 3 mM Na₂CO₃, pH 9.9 ± 0.1.

Methanol can be added to the buffer at a final concentration of 10-20%. This buffer may be used with or without the addition of SDS (0.01-0.1 %). Please see our 10% SDS and 20% SDS stock solution, sold separately.

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.

The buffer is Sterile filtered through 0.22 µm membrane, and autoclaved.

The buffer is evaluated and certified for the absence of DNase, RNase, and protease activities.

PACKAGING:

Available in different bottle sizes: 250 mL, 500 mL, and 1000 mL

Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact us with your request for customized packaging.

STORAGE TEMP

Room Temperature.

SHELF LIFE

2 years.

NOTES

Molecular Biology / Electrophoresis grade buffer, filter sterile, and DNase/RNase and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 4 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.