DESCRIPTION
The 10X FGRB (Formaldehyde-RNA Gel Running Buffer) is used as a running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels.
The buffer is also used for Northern Blots (hybridization of total RNA or mRNA to a membrane).
The 10X concentrated FGRB solution is made of MOPS (3-(N-morpholino) propanesulfonic acid). The 10X Buffer is composed of 200 mM MOPS, 100 mM Sodium Acetate, and 10 mM EDTA (pH 7.0 ± 0.1).
To use, dilute 1 volume of the buffer with 9 volumes of high-quality RNase-free deionized water, or DEPC treated water (See our Ultra-pure water section). The concentrations in 1X buffer solutions are: 20 mM MOPS, 10 mM Sodium Acetate, and 1 mM EDTA (pH 7.0 ± 0.1).
Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water to ensure utmost quality.
The buffer is sterile, filtered through 0.2µm membrane.
10X FGRB is tested and certified for the absence of RNase/DNase and protease activities.
PACKAGING:
Available in different bottle sizes: 250 mL, 500 mL and 1L.
Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.
Minimum order: 1 Bottle.
We accommodate customized packaging requests. Please contact our team, we fulfill most requests!
STORAGE TEMP
Room Temperature.
SHELF LIFE
2 years.
NOTES
Molecular biology grade buffer, filter sterile, and DNase/RNase and protease free.
Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.
Minimum order: 1 bottle.
Request a discounted quote for quantities ≥ 6 Bottles.
This product is developed, designed, and sold exclusively for research purposes and in vitro use only.
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