50X FGRB – Formaldehyde – RNA Gel Running Buffer (MOPS Buffer) Sterile Filtered, DNase/RNase Free (pH 7.0)

DESCRIPTION

50X FGRB (Formaldehyde-RNA Gel Running Buffer) is used as a running buffer to separate RNA samples on agarose and formaldehyde-agarose (denaturing) gels.

The buffer is also used for Northern Blots (hybridization of total RNA or mRNA to a membrane).
The concentrated FGRB solution is made of MOPS (3-(N-morpholino) propanesulfonic acid). The 50X Buffer is composed of 1 M MOPS, 0.5 M Sodium Acetate, and 50 mM EDTA (pH 7.0 ± 0.1).

To use, dilute 1 volume of the buffer with 49 volumes of high-quality RNase-free deionized water, or DEPC treated water (See our Ultra-pure water section). The concentrations in 1X buffer solutions are: 20 mM MOPS, 10 mM Sodium Acetate, and 1 mM EDTA (pH 7.0 ± 0.1).

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water to ensure utmost quality.

The buffer is sterile, filtered through 0.2µm membrane.

Each lot of the 50X BFGRB is tested and certified for the absence of RNase/DNase activities.

PACKAGING:

Available in different bottle sizes: 125 mL, 250 mL, 500 mL and 1L.

Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact our team, we fulfill most requests!

STORAGE TEMP

Room Temperature.

SHELF LIFE

2 years.

NOTES

Molecular biology grade buffer, filter sterile, and DNase/RNase and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 6 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.