Hybridization Denaturing Solution

DESCRIPTION

The Hybridization Denaturing Solution is a strong alkaline that denatures double-stranded DNA allowing it to efficiently be transferred onto a nylon membrane and subsequently hybridize to a labeled probe. In addition, the denaturing solution can be used to hydrolyze (breakdown) the phosphodiester bond at depurinated sites of DNA, when used in a combination with a depurination solution, leading to more efficient transfer of large DNA in neutral Southern transfers.

Med Biosciences Hybridization denaturing solution is composed of molecular biology grade 0.5 N NaOH, and 1.5 M NaCl. The solution is prepared with Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water to ensure utmost quality. The final solution is filtered using a 0.22 µm filter into final sterile bottles.

The solution is manufactured according to strict quality control standards to ensure lot-to-lot consistency. Our solution is certified sterile, DNase/RNase free, and protease free.

Each lot of our Molecular Biology Grade denaturing solution is subjected to rigorous quality control which involves checking for RNase activity, Exonuclease activity, Nonspecific Endonuclease activity, Nonspecific Protease activity, and Sterility.

Available in different convenient sizes (250 mL to 1 Gal sterile bottles). Customized solutions are available to meet individual needs.

PACKAGING:

Available in 250 mL, 500 mL, 1 L, and 1 Gal (3.78 L) bottles.

Hybridization Denaturing Solution is packaged in Sterile and RNase/DNase-free thick wall bottles that are classified for medical/food usage.

Minimum order: 1 bottle.

We accommodate customized packaging requests. Please contact our team, we fulfill most special requests!

STORAGE TEMP

Room Temperature.

SHELF LIFE

2 years.

NOTES

Caution: the solution contains 0.5N Sodium Hydroxide. Avoid skin, eye, clothing contact!

Each batch of Hybridization Denaturing Solution undergoes strict quality control and standard operating procedures to ensure the high quality of the final product, sterility, and lot-to-lot consistency.

Request a discount price offer for orders ≥ 4 bottles of any size.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.