0.5% Phenol Red Solution – Cell Culture Grade Solution

DESCRIPTION

Phenol red (also known as phenolsulfonphthalein or PSP) is used as a pH indicator. The sodium salt form of phenol red is used for preparation of cell culture media as a visual indicator to monitor shifts from neutral to acidic pH values. It is typically used in cell culture media where ideally a solution of phenol red will have a yellow to orange color at a pH ≤ 6.8, and a gradual increase in red color at pH 7.0 to reach dark red/purple color at pH ≥ 8.2 (please see the chart photograph for reference colors).

Med Biosciences 0.5% Phenol Red Solution is ready-to-use for Routine cell culture work and can be easily diluted to the desired lower concentrations.

Our Phenol Red solutions is prepared from a highly pure reagent with Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water to ensure utmost quality.

The solution is manufactured according to strict quality control standards to ensure lot-to-lot consistency. Our Phenol Red is Certified Sterile, DNase & RNase Free, Protease Free and Endotoxins Free (<0.0050EU/ml) solution. Each lot of our Cell Culture Grade 0.5% Phenol Red Solution is subjected to rigorous quality control validation which involves checking for Endotoxin lipopolysaccharides (LPL), RNase activity, Exonuclease activity and Nonspecific Endonuclease activity, Nonspecific Protease activity and Sterility as shown in the following table.

Test	Sample Incubated with Phenol Red Solution	Analysis After Incubation	Validation
Endotoxin contamination	Limulus amebocyte lysate	Photometric LAL quantitative test	Pass/Endotoxin Level <0.0050EU/ml
RNase activity	Labeled RNA	Electrophoresis (PAGE Analysis)	Pass/No Degradation
Exonuclease activity	Linear Double Stranded DNA	Electrophoresis (PAGE Analysis)	Pass/ No Degradation
Nonspecific Endonuclease Activity	Supercoiled plasmid DNA	Agarose Gel Electrophoresis	Pass/No Decrease Supercoiling
Nonspecific Protease Activity	Purified casein solution	Spectrophotometrically for liberated Tyrosine (tyr) residues from digested casein using Folin-Ciocalteus Phenol reagent (FC)	Pass/No Significant Blue Chromogen formation between Tyr and FC reagent.
Sterility	Microbial Growth after plating Phenol Red solution on specific microbiology agar plates (trypticase Soy Agar, and Nutrient Agar) and incubating for 48 hours at 37 ᴼC	Counting colony forming units on cultured plates (CFU)	Negative/No Colony Formation

Available in 2 convenient sizes (125 mL, and 250 mL sterile bottles). Customized solutions are available to meet individual needs.

COMPOSITION:

0.5% (5.0 g/L) solution is formulated from a Cell Culture Grade Phenol Red Sodium Salt [Molecular Weight: 376.36, Cas Number: 34487-61-1, highly pure, suitable for cell culture, suitable for insect cell culture] in Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water, pH 7.2-7.4.

PACKAGING:

Available in 125 mL, and 250 mL bottles.

0.5% Phenol Red solution is packaged in Sterile and RNase/DNase-free thick wall bottles that are made from medical/food-grade PET/PETG plastic materials.

Minimum order: 1 bottle.

We accommodate customized packaging requests. Please contact our team, we fulfill most requests!

STORAGE TEMP

2-25 ᴼC. Store at 2-8 ᴼC for extended shelf life.

SHELF LIFE

2 years if not opened.

NOTES

Important, phenol red in tissue culture media can function as a weak estrogen, a property associated with the presence of lipophilic impurities, especially observed in human breast cancer cells.

Do not autoclave the solution.

Each batch of Phenol Red solution undergoes strict quality control and standard operating procedures to ensure the high quality of the product, and lot-to-lot consistency.

Request a discount price offer for orders ≥ 6 bottles of any size.