1 M HEPES Buffer (pH 7.0, pH 7.2, pH 7.4, pH 7.5, pH 7.6, pH 7.8, and pH 8.0). Molecular Biology & Cell Culture Grade

DESCRIPTION

HEPES has been described as one of the best all-purpose buffers available for biological research. HEPES belongs to the zwitterionic Good buffer group and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55).

HEPES is ideal for cell culturing because of its usefulness in controlling pH changes in presence of carbon dioxide production from cellular respiration. It is also used for freezing protein solutions. The increase in acidity because of freezing observed in many buffers is negligible in the presence of HEPES buffer. HEPES protects enzymes and coenzymes from denaturation and inactivation during freeze-drying. HEPES buffer has a large list of applications in Molecular Biology, Cell Biology, and Immunology research.

Med Biosciences 1.0 M HEPES buffer meets the research needs in most applications:

1. The buffer is provided in different pH from pH 7.0 to pH 8.0 (pH 7.0, 7.2, 7.4, 7.5, 7.6, 7.8 & 8.0).

2. We use ultrapure molecular biology grade HEPES, and the 1.0 M solution is prepared in Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water to ensure utmost quality. Therefore, our buffer is suitable for all molecular biology applications.

3. Our buffer is sterile, filtered through 0.22 µm filter into a final sterile and DNase/RNase-free bottle. Therefore, there is no need to sterilize by filtration when you dilute the buffer using sterile water.

4. Our HEPES buffer is DNase/RNase and Protease free. The buffer is also Endotoxin-free (Endotoxin Level <0.0050EU/ml, determined by using Limulus amebocyte lysate). Therefore, our buffer is suitable for cell culture applications, DNA and RNA molecular analysis, biochemical and protein purifications and storage, cell imaging, staining and flow cytometry studies, and various immunological applications. 5. Each lot of our Molecular and Cell Culture Grade 1.0 M HEPES Solution is subjected to rigorous quality control which involves checking for Endotoxin lipopolysaccharides (LPL), RNase activity, Exonuclease activity and Nonspecific Endonuclease activity, Nonspecific Protease activity and Sterility as shown in the following table.

Test	Sample Incubated with HEPES Solution	Analysis After Incubation	Validation
Endotoxin contamination	Limulus amebocyte lysate	Photometric LAL quantitative test	Endotoxin Level <0.0050EU/ml
RNase activity	Labeled RNA	Electrophoresis (PAGE Analysis)	Pass/No Degradation
Exonuclease activity	Linear Double Stranded DNA	Electrophoresis (PAGE Analysis)	Pass/ No Degradation
Nonspecific Endonuclease Activity	Supercoiled plasmid DNA	Agarose Gel Electrophoresis	Pass/No Decrease Supercoiling
Nonspecific Protease Activity	Purified casein solution	Spectrophotometrically for liberated Tyrosine (tyr) residues from digested casein using Folin-Ciocalteus Phenol reagent (FC)	Pass/No Significant Blue Chromogen formation between Tyr and FC reagent.
Sterility	Microbial Growth after plating HEPES solution on specific microbiology agar plates (trypticase Soy Agar) and incubating for 48 hours at 37 OC	Counting colony forming units on cultured plates (CFU)	Negative/No Colony Formation

COMPOSITION:

1.0 M HEPES (238.30 g/L) in Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water.

SPECIFICATIONS:

Description	1.0 M HEPES [pH 7.0, 7.2, 7.4, 7.5, 7.6, 7.8, or 8.0 (see different Cat. No.)]. pH Tolerance is ± 0.1.
Synonyms	4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid).
Purity	≥99.5% (titration). Certified.
Grade	Cell Culture and Molecular Biology Grade
CAS Number	7365-45-9
EC Number	230-907-9
Empirical Formula	C8H18N2O4S
Molecular Weight	238.30
Physical State	Colorless Liquid (sterile filtered). Slight coloration with time will not affect the quality of this product.
Concentration	1.0 M
Application	buffer at pH 6.8 to 8.2 (pKa 7.55).
Preservative	None
Shipping Condition	Ambient
Storage Condition	Store container at room temperature (18 to 25 ᴼC) prior to opening. If desired, the solution may be stored at 2-8 ᴼC for extended shelf life.

PACKAGING:

Available in 125 mL, 250 mL, and 500 mL bottles. Different pHs have different catalog numbers.

1.0 M HEPES Buffer solution is packaged in Sterile and RNase/DNase-free thick wall bottles that are made from medical/food-grade PET/PETG plastic materials.

Minimum order: 1 bottle.

We accommodate customized packaging requests. Please contact our team, we fulfill most requests!

STORAGE TEMP

2-25 ᴼC. HEPES buffer can be stored at room temperature.

SHELF LIFE

24 months from manufacturing date.

NOTES

Each batch of the HEPES solution undergoes strict quality control and standard operating procedures to ensure the high quality of the final product, sterility, and lot-to-lot consistency.

Request a discount price offer for orders ≥ 4 bottles of any size.