DESCRIPTION
10X Phosphate-Buffered Saline with 0.5% Tween-20 (10X PBST) is a 10-fold concentrated buffer solution for different Western Blot and ELISA applications.
The 10X buffer solution is composed of 100 mM Sodium Phosphate Dibasic, 18 mM Potassium Phosphate Monobasic, 27 mM Potassium Chloride, 1.37 M Sodium Chloride, and 0.5% Tween-20 (pH 7.4 ± 0.1).
To use, dilute 1 volume of the buffer with 9 volumes of deionized water. The concentrations in 1X buffer solution are: 10 mM Sodium Phosphate Dibasic, 1.8 mM Potassium Phosphate Monobasic, 2.7 mM Potassium Chloride, 137 mM Sodium Chloride, and 0.05% Tween-20 (pH 7.4 ± 0.1). It is recommended to check the pH after dilution.
Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.
The buffer is Sterile, autoclaved (Heated to 121°C (15 psi) for 30 minutes on liquid cycle) and filtered through 0.22µm membrane.
The buffer is evaluated and certified for being RNase/DNase and protease free.
PACKAGING:
Available in different bottle sizes: 500 mL, 1000 mL, and 1 Gallon (3.78L).
Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.
Minimum order: 1 Bottle.
We accommodate customized packaging requests. Please contact us with your request for customized packaging.
STORAGE TEMP
Room Temperature
SHELF LIFE
2 years
NOTES
Molecular Biology grade buffer, filter sterile, and DNase/RNase and protease free.
Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.
Minimum order: 1 bottle.
Request a discounted quote for quantities ≥ 3 Bottles.
This product is developed, designed, and sold exclusively for research purposes and in vitro use only.
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