DESCRIPTION
10X Towbin Tris-Glycine-SDS (with 0.5% SDS) is a standard transfer buffer for Western blots.
This transfer buffer is optimal for tank (wet) blotting and for some semi-dry apparatuses.
The presence of SDS in the 1X Towbin buffer can increase the efficiency of transfer of medium and large proteins.
The presence of SDS increases the ionic strength of the blot buffer which will increase heating during the transfer process; therefore, it is recommended to use ice packs in the transfer tank.
The 10X Towbin SDS buffer is composed of 250 mM Tris-Base, 1.92 M glycine, and 0.5% SDS (pH 8.3 ± 0.1).
To make 1X solution, dilute 10-fold with deionized water and the appropriate volume percentage of methanol. The concentrations in 1X solutions are: 25 mM Tris-Base, 192 mM Glycine, and 0.05% SDS (pH 8.3 ± 0.1).
Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.
The buffer is Sterile filtered through 0.22 µm membrane, and RNase/DNase & Protease-free.
PACKAGING:
Available in different bottle sizes: 500 mL, 1000 mL, and 1 Gallon (3.78 L).
Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.
Minimum order: 1 Bottle.
We accommodate customized packaging requests. Please contact us with your request for customized packaging.
STORAGE TEMP
Room Temperature.
SHELF LIFE
2 years.
NOTES
Molecular Biology / Electrophoresis grade buffer, filter sterile, and RNase/DNase & protease free.
Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.
Minimum order: 1 bottle.
Request a discounted quote for quantities ≥ 4 Bottles.
This product is developed, designed, and sold exclusively for research purposes and in vitro use only.
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