10X TT-SDS – Tris-Tricine-SDS Running Buffer (pH 8.3)

DESCRIPTION

The 10X Tris-Tricine-SDS (TT-SDS) Running Buffer is used with Tris-Tricine gel electrophoresis. Tris-Tricine gels are specifically designed for separating peptides and small proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and de-stacking of low molecular weight proteins (1Kd to 40Kd) and higher resolution of smaller peptides.

Med Biosciences Tris-Tricine-SDS running buffer is compatible with both home-made and commercial precast Tris-Tricine gels such as Mini-PROTEAN^® and midi Criterion™ gels from BioRad, Invitrogen Novex Tricine Gel from ThermoFisher Scientific, TEO Tricine Precast Gels from Abcam, and other commercially available Tris-Tricine precast gels.

The 10X concentrated TT-SDS buffer is composed of 1 M Tris-Base, 1 M Tricine, 1% SDS (pH 8.3 ± 0.2).

To prepare a 1X solution, Dilute 1 volume of the buffer with 9 volumes of deionized molecular grade water. The final concentrations in 1X solution: 100 mM Tris, 100 mM Tricine, 0.1% SDS, (pH 8.3 ± 0.2). It is recommended to check the pH of the solution after dilution.

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.

The buffer is Sterile, filtered through 0.22 µm membrane.

The buffer is evaluated and certified for being RNase/DNase and protease free.

PACKAGING:

Available in 2 different bottle sizes: 500 mL and 1000 mL.

Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact us with your request for customized packaging.

STORAGE TEMP

Room Temperature.

SHELF LIFE

2 years.

NOTES

Molecular/Electrophoresis grade buffer, filter sterile, and DNase/RNase and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 3 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.