DESCRIPTION
1X Towbin Tris-Glycine (with 20% Methanol) is the standard transfer buffer for Western blots.
This transfer buffer has both low ionic strength and low conductivity, which are optimal for tank (wet) blotting and for some semi-dry apparatuses.
The 1X Towbin buffer is composed of 25 mM Tris-Base, 192 mM glycine, and 20% Methanol (pH 8.3 ± 0.1). This buffer does not contain SDS.
Sometimes SDS is added to this buffer, in the range of 0.025% to 0.1% to improve transfer of large-size proteins (see our buffers with Cat. No. BTOWT-ME025S-1X, BTOWT-ME05S-1X, and BTOWT-ME1S-1X).
Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.
The buffer is Sterile filtered through 0.22 µm membrane.
The buffer is evaluated and certified for being RNase/DNase and Protease-free.
PACKAGING:
Available in different bottle sizes: 1000 mL, and 1 Gallon (3.78 L).
Our thick wall bottles are leak-proof and made from Medical/Food grade PET and PETG.
Minimum order: 1 Bottle.
We accommodate customized packaging requests. Please contact us with your request for customized packaging.
STORAGE TEMP
Room Temperature.
SHELF LIFE
2 years.
NOTES
Molecular Biology / Electrophoresis grade buffer, filter sterile, and protease free.
Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.
Minimum order: 1 bottle.
Request a discounted quote for quantities ≥ 3 Bottles.
This product is developed, designed, and sold exclusively for research purposes and in vitro use only.
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