2.0 M Imidazole Buffer Solution (pH 6.5, pH, pH 6.8, pH 7.0, pH 7.5, and pH 7.9) – Molecular Biology Grade Solution

DESCRIPTION

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25 OC. It is recommended as a buffer component for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Med Biosciences 2.0 M Imidazole Buffer Solution is provided at different pH from pH 6.5 to pH 7.9.

We use ultrapure molecular biology grade imidazole, and the 2.0 M solution is prepared with Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water to ensure utmost quality. Our buffer is sterile, filtered through 0.22 µm filter into a final sterile and DNase/RNase-free bottle. The buffer is certified being DNase/RNase-free, Protease free, and phosphatase free. Therefore, our buffer is suitable for most molecular biology applications.

Each lot of our Molecular Biology Grade Imidazole buffer is subjected to rigorous quality control which involves checking for RNase activity, Exonuclease activity, Nonspecific Endonuclease activity, Nonspecific Protease activity, phosphatase activity, and Sterility as shown in the following table.

Test	Sample Incubated with Imidazole Solution	Analysis After Incubation	Validation
RNase activity	Labeled RNA	Electrophoresis (PAGE Analysis)	Pass/No Degradation
Exonuclease activity Nonspecific Endonuclease Activity	Linear Double Stranded DNA Supercoiled plasmid DNA	Electrophoresis (PAGE Analysis) Agarose Gel Electrophoresis	Pass/ No Degradation Pass/No Decrease Supercoiling
Nonspecific Protease Activity	Purified casein solution	Spectrophotometrically for liberated Tyrosine (tyr) residues from digested casein using Folin-Ciocalteus Phenol reagent (FC)	Pass/No Significant Blue Chromogen formation between Tyr and FC reagent.
Nonspecific Phosphatase	p-nitrophenyl phosphate	Spectrophotometrically for the generated p-nitrophenol after removing the phosphate group by the potential phosphatases.	Pass/No Significant yellow Chromogen formation due to liberation of p-nitrophenol
Sterility	Microbial Growth after plating imidazole solution on specific microbiology agar plates, such as trypticase Soy Agar and nutrient Agar and incubating for 48 hours at 37 ᴼC	Counting colony forming units on cultured plates (CFU)	Negative/No Colony Formation

COMPOSITION:

2.0 M Imidazole (137.16 g/L) in Autoclaved 18.2 mega Ω-cm DNase/RNase-free Water. The buffer is available in pH 6.5 to pH 7.9 ± 0.1 at 25 ᴼC. The final solution is filtered through 0.22 µm filter into the final sterile, DNase/RNase free bottles.

SPECIFICATIONS:

Description	2.0 M Imidazole Buffer Solution [pH 6.5, 6.8, 7.0, 7.5, or 7.9 (see different Cat. No.)]. pH tolerance is ± 0.1 at 25 ᴼC
Synonyms	Imidazole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
Chemical Purity	for molecular biology, ≥99% (titration)
Grade	Molecular Biology Grade, Sterile, DNase/RNase free, Protease Free, Phosphatase free, and filtered through 0.22 µm filter.
CAS Number	288-32-4
Empirical Formula	C3H4N2
Molecular Weight	68.08
Physical State	Colorless Liquid (sterile filtered)
Concentration	2.0 M N/A
Application	Useful buffer at pH 6.2-7.8 (pKa 6.95).
Preservative	None
Shipping Condition	Ambient
Storage Condition	Store container at 2 to 25 ᴼC) prior to opening. The solution may be stored at 2-8 ᴼC for extended shelf life.

PACKAGING:

Available in 125 mL, 250 mL, and 500 mL bottles. Different pHs have different sub-catalog numbers.

2.0 M Imidazole Buffer solution is packaged in Sterile and RNase/DNase-free thick wall bottles that are made from medical/food-grade PET/PETG plastic materials.

Minimum order: 1 bottle.

We accommodate customized packaging requests. Please contact our team, we fulfill most requests.

STORAGE TEMP

2-25 ᴼC. room temperature if not opened. Store at 2-8 ᴼC for extended shelf life.

SHELF LIFE

1 year from manufacturing date.

NOTES

The pH values of all buffers are temperature and concentration dependent. For Imidazole, pH increases about 0.02 unit per 1 ᴼC decrease in temperature and decreases 0.02-0.04 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Each batch of the Imidazole buffer solution undergoes strict quality control and standard operating procedures to ensure high quality and sterility.

Request a discount price offer for orders ≥ 4 bottles of any size.