2X Tricine SDS Sample Buffer (W/O Reducing Agent)

DESCRIPTION

Tricine SDS Sample Buffer is used to prepare protein samples for denaturing gel electrophoresis using Tris-Tricine denaturing PAGE for separating peptides and small size proteins (˂40kD).

The Tricine sample buffer is formulated based on the method of Schagger von Jagow (1987) with special modifications in our laboratory to grant optimal band resolution and bands tightness when preparing peptides and small proteins for denaturing PAGE with Tris-Tricine-SDS running buffer (please see our products Cat. No. BTTS70-10X and Cat. No. BTTS70-1X).

Tricine-SDS sample buffer is compatible to prepare samples for loading on both home-made and precast Tris-Tricine gels (ready-made) such as Mini-PROTEAN^® and midi Criterion™ gels from BioRad, Invitrogen Novex Tricine Gel from ThermoFisher Scientific, TEO Tricine Precast Gels from Abcam, and other commercially available Tris-Tricine precast gels.

The 2X Tricine-SDS sample buffer is composed of 200 mM Tris-HCl, 40% glycerol, 2% SDS, and 0.04% Coomassie Blue G-250 (pH 6.8).

Our sample buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.

Confirmed test for being RNase/DNase and protease free.

PROCEDURE:

Add a Reducing Agent: add either 20 µl of β-mercaptoethanol per 950 µl of the 2X sample buffer (final concentration 2% [284 mM]), or 19.3 mg dithiothreitol (DTT) per 1 mL of the sample buffer (final concentration of DTT in the 2X sample buffer is 125 mM). Note: For best results, prepare fresh reducing sample buffer prior to use.

If reduction of proteins is not needed, then you can exclude the above step and proceed to the next step.

Add the reducing buffer to samples: Dilute 1 part sample with 1 part of the reducing or non-reducing sample buffer. More sample buffers can be added if necessary. Dry samples can be dissolved directly into the 2X reducing sample buffer. Then, Heat the sample/sample buffer mixture at 85 ᴼC for 2 minutes for optimal results. Centrifuge for 2 minutes to precipitate undissolved materials and load the samples on the gel.

PACKAGING:

Available in 3 different sizes: 10 mL, 25 mL, and 50 mL.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact us with your request for customized packaging.

STORAGE TEMP

Room Temperature.

SHELF LIFE

1 years.

NOTES

Molecular/Electrophoresis grade buffer, DNase/RNase, and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 4 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.