6X Laemmli SDS-Sample Buffer (+ Reducing Agent)

DESCRIPTION

The 6X Laemmli SDS-Sample Buffer with the reducing agent 2-mercaptoethanol (β-mercaptoethanol) is used for the preparation of samples for SDS PAGE.

Laemmli SDS sample buffer is used as an electrophoretic dye for the denaturation of proteins and monitoring of the front of running gel.

Our 6X Laemmli Sample Buffer can be used with both home-made and commercially available precast protein gels such as Mini-PROTEAN® and midi Criterion™ gels manufactured by BioRad, Novex Tris-Glycine gels from ThermoFisher, and many others.

The 6X SDS-Sample buffer is composed of 375 mM Tris-HCl, 9% Sodium Dodecyl Sulfate (SDS), 50% Glycerol, 0.075% Bromophenol Blue, and 9% Beta-Mercaptoethanol (pH 6.8 ± 0.1).

The final concentrations in 1X SDS Sample buffer are: 62.5 mM Tris Base / Tris-HCl, 1.5% Sodium Dodecyl Sulfate (SDS), 8.33% Glycerol, 0.0125% Bromophenol Blue, and 1.5% Beta-Mercaptoethanol (pH 6.8 ± 0.2).

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water.

The sample buffer is evaluated and certified for the absence of RNase/DNase and protease activities.

PROCEDURRE:

Dilute 1 volume of the 6X sample buffer with 5 volumes of sample solution (Example: add 5 µL of the 6X sample buffer to each of the 25 µL samples).

For dry or precipitated protein samples, dilute the sample buffer to 1X solution using deionized water. Then dissolve the protein sample in an appropriate amount of the diluted sample buffer.

Samples are heated in the gel loading/sample buffer for either 5 minutes at 100 ᴼC, or 10 minutes at 70 ᴼC to aid in the denaturation of proteins. At this point, samples may remain at room temperature if they are to be used immediately or stored at either 4 ᴼC or –20 ᴼC for later analysis.

PACKAGING:

Available in 4 different sizes: 1.5 mL, 10 mL, 25 mL, and 50 mL Bottles.

Minimum order: 1 Bottle.

We accommodate customized packaging requests. Please contact us with your request for customized packaging.

STORAGE TEMP

2-15 ᴼC.

SHELF LIFE

1 years.

NOTES

Molecular/Electrophoresis grade buffer, DNase/RNase, and protease free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality of the final product.

Minimum order: 1 bottle.

Request a discounted quote for quantities ≥ 4 Bottles.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.