6X Orange DNA Sample Loading Buffer

DESCRIPTION

The 6X DNA Sample Loading Buffer (Dye) is used for loading DNA markers and DNA samples on agarose or polyacrylamide gels.

Med Biosciences DNA sample buffer is ideal for improving resolution and separation of DNA fragments.

The sample buffer contains two tracking dyes, Orange G and Xylene Cyanol FF, for visual tracking of DNA migration during agarose gel electrophoresis.

In 1% agarose gels Orange G comigrates with a ~50 bp DNA fragment and Xylene Cyanol FF – with a ~4000 bp fragment.
The 6X DNA Sample Loading Buffer is composed of 10 mM Tris-HCl, 0.15% Orange G, 0.03% Xylene Cyanol FF, 60% Glycerol, and 60 mM EDTA (pH 7.6).

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water to ensure utmost quality.

Our buffer is tested and certified for the absence of DNase and RNase activity.

PROCEDURE:

Add 1 volume of the 6X loading buffer to each 5 volumes of your sample and mix well before loading on gels.

PACKAGING:

5x 500 µL vials
5x 1 mL vials
4x 5 mL vials

We accommodate customized packaging requests. Please contact our team, we fulfill most of customized requests!

STORAGE TEMP

Room temperature or 4 ᴼC.

SHELF LIFE

Minimum 1 year when stored at room temperature or 4 ᴼC. for longer periods (minimum 3 years), store at -20 ᴼC

NOTES

Molecular biology grade buffer, sterile, and DNase/RNase free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure the high quality of the final product and lot-to-lot consistency.

Request a discounted quote for quantities ≥ 20x 500 µL, 20x 1 mL, or 16x 5 mL.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.