RNA Sample Loading Buffer – for RNA Electrophoresis [With Ethidium Bromide 50 μg/mL)] – Sterile Filtered, DNase/RNase Free

DESCRIPTION

The RNA Sample Loading Buffer is formulated to be used in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis.

Med Biosciences RNA sample buffer is suitable for use in formaldehyde-agarose gel electrophoresis of RNA. The solution contains tracking dyes and ethidium bromide; therefore, ethidium bromide gel staining is not required after electrophoresis.

The RNA Sample Loading Buffer contains the electrophoresis tracking dyes bromophenol blue, xylene cyanol FF, and the intercalating dye ethidium bromide. In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than the human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than the 18S rRNA.

The sample loading buffer is composed of 62.5% deionized Formamide, 1.14 M Formaldehyde, 1.25X MOPS-EDTA-Sodium Acetate buffer (Cat. No. BFGRB-10X-250ML, diluted 1:8), 200 µg/ml Bromophenol blue, 200 µg/ml Xylene Cyanole FF, and 50 µg/ml Ethidium Bromide.

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water to ensure utmost quality.

The buffer is sterile, filtered through 0.2µm membrane.

RNA sample buffer is tested and certified for the absence of DNase and RNase activity.

PROCEDURE:

Add the loading buffer to your sample in the ratio of 2:1 to 5:1 and mix well.

Just before loading, heat the samples at 65 ᴼC for 10 minutes, then chill on ice and spin down prior to loading on a gel.

PACKAGING:

5x 500 µL vials
5x 1 mL vials
4x 5 mL vials

We accommodate customized packaging requests. Please contact us, we fulfill most customized orders.

STORAGE TEMP

-20 ᴼC.

SHELF LIFE

2 years.

NOTES

Molecular biology grade buffer, filter sterile, and DNase/RNase free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure the high quality of the final product and lot-to-lot consistency.

This RNA sample loading buffer contains ethidium bromide which is not recommended for gel staining prior to Northern blot detection, because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer. For Northern blotting, use our RNA sample loading buffer that does not have ethidium bromide (Cat. No. RNASB02-5X500µL, RNASB02-5X1ML)

Request a discounted quote for quantities ≥ 20x 500 µL or 20x 1 mL.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.