RNA Sample Loading Buffer (w/o Eth. Br.) – for RNA electrophoresis and Northern Blot, without Ethidium Bromide – Sterile Filtered, DNase/RNase Free

DESCRIPTION

The RNA Sample Loading Buffer is formulated to be used in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis.

This buffer is suitable for use in Northern blots. The presence of ethidium bromide in agarose gels or the loading buffer has been shown to cause poor transfer efficiency and can affect the length of transfer time.

The RNA loading dye has a slight negative charge and will migrate in the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel.

The sample loading buffer is composed of 62.5% deionized Formamide, 1.14 M Formaldehyde, 1.25X MOPS-EDTA-Sodium Acetate buffer (Cat. No. BFGRB-10X-250ML, diluted 1:8), 200 µg/ml Bromophenol blue, and 200 µg/ml Xylene Cyanole FF.

Our buffer is formulated from Highly Pure Molecular-Grade Chemicals in Autoclaved 18.2 mega Ω-cm Water to ensure utmost quality.

The sample buffer is sterile, filtered through 0.2µm membrane.

Our RNA sample buffer is tested and certified for the absence of DNase and RNase activity.

PROCEDURE:

Add the loading buffer to your sample in the ratio of 2:1 to 5:1 and mix well.

Just before loading, heat the samples at 65 ᴼC for 10 minutes, then chill on ice and spin down prior to loading on a gel.

PACKAGING:

5x 500 µL vials
5x 1 mL vials
4x 5 mL vial

We accommodate customized packaging requests. Please contact our team, we fulfill most customized requests.

STORAGE TEMP

-20 ᴼC.

SHELF LIFE

2 years.

NOTES

Molecular biology grade buffer, filter sterile, and DNase/RNase free.

Each batch of buffers undergoes strict quality control and standard operating procedures to ensure high quality and lot-to-lot consistency.

Request a discounted quote for quantities ≥ 20x 500 µL or 20x 1 mL.

This product is developed, designed, and sold exclusively for research purposes and in vitro use only.