DESCRIPTION
Med Biosciences Virar Transport Medium Powder Mixture is our most effective economy option for preparing VΤM. We have 2 size options for the VΤM power mixture; one for making 25 L of medium and the other for 50 L. save time and efforts of preparation, just reconstitute the chemical mixture with molecular grade water and 2% Fetal Bovine Serum, sterilize by filtration through 0.22 µm filter, and aseptically dispense in sterile and DNase/RNase-free tubes. We recommend using our long 10 ml tubes (Cat. No. CV-10-PP), or our shot 5 mL tubes (Cat. No. CV-5-PP). Please see under consumables.
VΤM powder mixture is ideal for long distance shipping with minimum cost, in addition to the extended shelf life of 5 years for the medium.
Med Biosciences (VΤM) Viral Transport Medium provides an appropriate transport and storage environment for clinical sampled swabs. Our VΤM is intended for transporting clinical specimens suspected to contain SARS-CoV-2 (COVID-19) for subsequent testing using biochemical, molecular assays (RNA extraction and PCR/RT-PCR), antigen assays, or other validated diagnostic protocols.
The Medium is formulated following the United States Center of Disease Control CDC SOP# DSR-052-05, Preparation of Viral Transport Medium under FDA device listing D411652 (Code JSG, Exempt).
Med Biosciences (MedBio, LLC.) is authorized by FDA to commercially manufacture and distribute Viral Transport Medium VΤM as set forth in Section IV.B of the FDA’s COVID-19 Transport Media Policy.
Our VΤM powder mixture is packaged in sealed special plastic bottles to prevent potential moisture and light exposures. We provide two options for the medium powder: without or with separately packaged Heat inactivated fetal bovine serum (FBS) bottles. We recommend using our high-quality heat inactivated FBS, which we obtain from a well-known US supplier (USDA approved Origin), and extensively tested for mycoplasma and other pathogens. In addition, each batch of our serum is extensively tested for efficacy with our reconstituted powder medium.
Our powder medium is produced under highest manufacturing standards. It is formulated from highly pure molecular-grade chemicals.
Each batch of our powder VΤM is extensively evaluated for efficacy and stability.
COMPOSITION:
After reconstituting the powder mixture in the indicated final volume of molecular grade water (including the volume of FBS, 20 ml/L), the final concentrations in 1X medium solution are 1X Hanks Balanced Salt Solution (HBSS), 2% Heat Inactivated FBS, 50 mg/mL Gentamicin Sulfate, 250 µg/mL Amphotericin B, and 0.0003% Phenol Red pH (7.3 ± 0.2).
The medium is provided as a powder mixture (containing HBSS, Gentamicin Sulfate, Amphotericin B, and Phenol Red) in a bottle + 1 x 500 mL heat inactivated FBS (for final volume of 25 L medium) and 2x 500 mL FBS (for final volume of 50 L medium).
PROCEDURE:
To prepare the medium, dissolve completely the contents of the powder mixture bottle in 22 L or 47 L of DNase/RNase-free deionized water, for the 25 L mixture and 50 L mixture, respectively. Then add 500 mL FBS (for the 25 L mixture), and 1 L FBS (for the 50 L mixture), adjust the pH to 7.3 ± 0.2 if necessary, and add water to the final volume of 25 L and 50 L, respectively (pH is 7.3 to 7.5). Finally, filter the medium using 0.22 µM filter cartridge, and aliquot 2 or 3 mL in sterile tubes and store at 2-8 ᴼC. non-aliquoted prepared medium should be also stored at 2-8 ᴼC.
PACKAGING:
The medium powder kit is provided in a sealed polypropylene bottle without and with 1x 500 mL or 2 x 500 mL heat inactivated bovine serum.
STORAGE TEMP
Store the Powder mixture and Serum bottles at -20 á´¼C. the powder mixture is stored in freezer because of its antibiotics content.
SHELF LIFE
Minimum 5 years for the powder medium at -20 á´¼C.
3 years for FBS at -20 to -80 á´¼C.
NOTES
Med Biosciences is authorized by FDA to Manufacture and distribute VΤM. The authorization can be accessed using the following link: https://www.fda.gov/medical-devices/coronavirus-covid-19-and-medical-devices/faqs-viral-transport-media-during-covid-19
Each batch of our powder VΤM is vigorously evaluated for efficacy and stability.
Powder medium and FBS are shipped in dry ice. Store at -20 á´¼C upon receiving.
Please contact us if you have special shipping or packaging requirement. We can accommodate most requests!
Request a discount price offer for orders ≥ 3 powder kits.
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